Chromatography & Putting It All Together

IN THIS COURSE

• 1.  Chromatography Part 1: Intro To Chromatography
  5m 32s
  In the following video, Dr. Barone discusses chromatography in more detail as well as explains how chromatography was invented. FREE ACCESS
• 2.  Chromatography Part 2: Chromatography Operation
  5m 44s
  In the following video, Dr. Barone discusses some theoretical aspects of chromatographic operation as well as some of the history behind their development. FREE ACCESS
• 3.  Chromatography Part 3: Gel Filtration Chromatography
  5m 50s
  In the following video, Dr. Barone discusses some key discoveries that enabled chromatography to be used to separate proteins. He also explains gel filtration in more detail. FREE ACCESS
• 4.  Chromatography Part 4
  6m 13s
  In this last segment about chromatography, Dr. Barone discusses each of the remaining methods in more detail and introduces some theoretical considerations when designing a chromatographic process. FREE ACCESS
• 5.  Capture Chromatography In Action
  3m 17s
  What does a chromatography column actually look like? Let's go find out. In the following video, Dr. Barone is back at Pfizer with Scientist Mark Kivimaki discussing affinity chromatography for protein purification. FREE ACCESS
• 6.  Polishing Chromatography In Action
  1m 23s
  In the steps of downstream processing, initial purification is followed by polishing. Polishing is often done using chromatography. In the following video, Dr. Barone and Pfizer's Mark Kivimaki go over the process of polishing chromatography for removal of impurities. FREE ACCESS
• 7.  Purification Of Insulin
  3m 53s
  Now that we have all of the tools of downstream processing, let's finish our example of purifying insulin. In the final segment on the purification of insulin, Dr. Barone analyzes the remaining steps needed to obtain a pure insulin product. FREE ACCESS
• 8.  Purification Of Monoclonal Antibody
  7m 11s
  In the following video, Dr. Barone walks through the downstream processing steps for a monoclonal antibody produced from mammalian culture. FREE ACCESS
• 9.  Viral Clearance In Downstream Purification
  6m 6s
  Dr. Barone discusses the role downstream processing plays in the inactivation and removal of potential viruses. FREE ACCESS