Cell Line Development

IN THIS COURSE

• 1.  Overview Of Cell Line Development
  4m 35s
  In the following video, Professor Love will talk about the steps in cell line development and highlight why choosing the correct host cell line has an impact on nearly all areas of manufacturing. FREE ACCESS
• 2.  History Of Recombinant DNA
  5m 15s
  In the following video, Professor Love tells you about the history behind the invention of recombinant DNA technology. FREE ACCESS
• 3.  Design Of DNA Vectors For Protein Expression
  4m 4s
  In the following video, Professor Love will discuss in more detail the common features of expression vectors used for heterologous protein expression. FREE ACCESS
• 4.  Selection Of A Host For Production: Part 1
  2m 58s
  In the following video, Professor Love discusses some of the key factors to consider when choosing a type of cell to manufacture a biologic drug. FREE ACCESS
• 5.  Selection Of A Host For Production: Part 2
  5m 23s
  In the following video, Professor Love will discuss these topics in more detail as well as describe the origin of one of the most common cell lines used in modern biopharmaceutical manufacturing, Chinese Hamster Ovary, or CHO, cells. FREE ACCESS
• 6.  Vector Design For Mammalian Hosts: Part 1
  6m 31s
  In the following video, Professor Love describes the elements of vector design in mammalian hosts in more detail. You will also hear from MIT Professor Phil Sharp about one of the first selectable markers used in mammalian cells. FREE ACCESS
• 7.  Vector Design For Mammalian Hosts: Part 2
  4m 37s
  In the following video, Professor Love will discuss these topics in more detail and show you examples of each strategy to improve protein production. FREE ACCESS
• 8.  Clonal Selection
  5m 50s
  In the following video, Professor Love discusses clonal selection topics in more detail. At the end, you will be able to identify important criteria for selecting an ideal clone. FREE ACCESS
• 9.  Cell Line Development In Action
  5m 58s
  Now that we know how to select our clones, let's find out what happens in a Cell Line Development lab. In the following video, Professor Love is on location at Pfizer in Andover, MA to talk to Principal Scientist Linda Francullo about the process that they use to develop their cells. FREE ACCESS
• 10.  Automation For Clonal Selection
  3m 18s
  In the following video, Professor Love visits the Pfizer Pilot Plant in Andover, MA. There, he talks to former Principal Scientist Tom Nelson (now Data Scientist, Computational Biology and Machine Learning at Biocomputronics) about automation for clonal selection. FREE ACCESS
• 11.  Cell Banking
  4m 1s
  In this video, Professor Love discusses both how to prepare, and how to thoroughly characterize, a master cell bank in more detail. FREE ACCESS
• 12.  Meet The Experts: Phillip A. Sharp
  6m 45s
  Meet a leading expert in the field: Phillip A. Sharp FREE ACCESS